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To perform the experiment using this assay in a manner allowing comparison with the in vivo data from periostin −/− mice, we should carry out an siRNA experiment.
However, previous experience using this assay in solid-organ transplant recipients indicates that this is unlikely to be the case, and we have reported the total lymphocyte counts, which also support this notion.
Future plans nationally in South Africa are to enhance the capacity for routine testing of all smear-positive adult and paediatric specimens using this assay, in order to report drug sensitivity patterns more rapidly.
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We have used this assay in conjunction with cocrystal structures of small molecules to drive a structure-based design program aimed at the discovery and optimization of a novel class of potent Hsp90 inhibitors.
Yatabe et al (2006) has developed a rapid assay to detect EGFR mutations, and we have decided to use this assay in a phase III trial.
We have used this assay in order to evaluate changes of the antioxidant capacity of patients during a cycle of hemodialysis.
Bansal and colleagues [ 32] used this assay in 46 trauma patients and showed that a large percentage of patients had undetectable or low platelet inhibition despite reported use of clopidogrel.
We also used this assay in uncovering a subset of anti-CCP− RA patients who have ACPAs, and in confirming that smoking and carriage of the HLA-DRB1 SE are associated with greater ACPA reactivity.
We have successfully used this assay in the high-throughput screening (HTS) of the NIH MLPCN small molecule library to discover small molecule inhibitors of Keap1 Nrf2 interaction 7, 102, 106.
Here, we used this assay in orthotopic xenografts of human MDA-MB-231 breast cancer cells to isolate selectively the migratory cell subpopulation of the primary tumor for gene-expression profiling.
A short list of some of the mediators that can be analyzed using this assay is shown in Table 1C.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com