We then used these strains to develop and validate a flow cytometry based assay for quantifying intracellular cryptococcal proliferation.
Since the GFP-positive strains described above are unaltered in their behaviour in macrophages, we used these strains to develop a flow cytometry-based methodology for quantifying intracellular proliferation of cryptococci after lysis of mammalian cells.
Because individuals from any given inbred strain can be replicated at will, it is possible to use these strains to precisely characterize as many phenotypes as desired, to determine the relationships between them, and to disentangle the contribution of both male and female factors to overall reproductive success.
Since the B. subtilis strains 6051, 23059, and 23856 were known to emit isoprene (Kuzma et al. 1995), we used these strains to set up the detection system for B. subtilis 168 for which the genome has been sequenced (Kunst et al. 1997) and functional knock outs have been made.
Since the only genetic differences in these unique animal models are those possibly existing between C57BL/6J and C57.YA, we used these strains to further test whether, in addition to affecting the size of CMs, the origin of chrY could also be linked to differences in the profile of gene expression in the hearts of adult male mice.
We further used these strains to test whether: 1) the origin of chrY could also be linked to differences in the profile of gene expression in the hearts of adult male mice, and 2) post-pubertal testosterone could play a role in the differential morphologic and/or molecular effects of chrYC57 and chrYA.
Using these strains, many different chemicals were tested and characterized.
Fluctuation assays were performed using these 43 strains to determine the mutation rate at the URA3 and CAN1 genes.
To use these strains as orally administered vectors to deliver other antigens we incorporated the B subunit of shiga-like toxin 1(Stx1) into the passenger domain of the autotransporter EspP expressed on a plasmid.
Thus, the findings may help us to beneficially use these strains and other related microbes in decolorizing and thereby detoxifying treatment of various dye containing effluents prior to discharge or reuse.
