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Using these protocols as case studies, we demonstrate previously unpublished flaws in these protocols.
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The use of these protocols as well as other care strategies initiated by the doctor was recorded every six months using a standardised form (see web extra).
The RNAs from FMH and JHU were labeled and hybridized to the array using the ATCC 23344 RNA as the reference using protocols as described.
However, many nurses emphasized the importance of using protocols as long as it did not inhibit their own critical thinking.
Spatial learning was tested using the protocol as described in Wood et al. [20].
The overpression, purification of His-tagged protein and enzymatic assays were carried out using the protocol as described in [36].
Next, treated cells were processed using the protocol as supplied by the manufacturer (Merck, Calbiochem, EMD Chemicals Inc., Darmstadt, Germany).
The cause of death was determined using the protocol as described by Patchell et al. [ 17].
Sequencing was performed with Ion-Torrent PGM at University of Pennsylvania, Philadelphia using the protocol as described [ 27].
Analyzes will be performed using both per protocol as well as intention to treat analyses.
Trained nurses conducted these tests using standardized protocols as described elsewhere.
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