We purified plasmid DNA from the bacterial transformants harboring the FLEX plasmids and used them to transform yeast strain Y6897 [ MATα his3 Δ1 leu2 Δ0 ura3 Δ0 met15 Δ0 can1 Δ:: STE2pr-Sp_his5 lyp1 Δ:: STE3pr- LEU2 (Tong and Boone 2006)], which is compatible with both SGA technology and the Barcoders collection (Yan et al. 2008).
We estimate the eigenvectors from just a fraction of the data, then use them to transform the data into approximately orthogonal series that deliver a well-conditioned estimator (by construction), even when there are fewer observations than dimensions.
Hence, we produced DNA constructs encoding secreted alkaline phosphatase (SEAP) under the transcriptional control of each of the four temporally distinct classes of baculovirus promoters, used them to transform insect cells, and compared the levels of SEAP RNA and protein production obtained before and after baculovirus infection.
+ Chilling cinemagraphs were Christiaan Welzel's medium of choice for capturing the creepiness of Chernobyl, while Jon Jacobsen used them to transform normal portraits into face-melting creatures.
And researchers are now realising that emoticons may hold a power over us that we didn't realise – when we use them to communicate, they can transform our decisions and behaviour in unexpected ways.
The aim is to aggregate them, to transform them or to use them to estimate other data.
A means of using style to transform yourself".
This post is part of "Being Yoga," a series on using yoga to transform your life.
Turner called them "painting days", however, and used them not to adjust but to transform his pictures and also to prove to himself and younger artists that he could still do it.
The dresses and make-up used to transform them into their true selves during the day are at night used to try and find clients, who often violently assault and rob them.
