Sentence examples for using the wild type from inspiring English sources
L-Galactonic acid production was not observed in either process using the wild type strain.
Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation).
Single amino acid mutations were introduced to the HBGA binding site of the P protein of BV through site-directed mutagenesis using the wild type construct of P domain (in plasmid pGEX-4T-1, GE Heastemplateife Sciences) as template.
To produce rhamnetin using enzymatic engineering, poplar O-methyltransferase-7 and its mutants were prepared based on the rational enzyme design, and the production of rhamnetin was compared with the results obtained using the wild type enzyme.
StaI and StaK were successfully produced in E. coli in the soluble fraction with high purity using the wild type gene for StaI and a synthetic codon optimized gene for StaK.
Another structure-based design of new nonphosphorylated substrates was focused on the aldol reaction with inversion in enantioselectivity using the wild type or the S238D variant as the catalyst and 2-methyl-substituted aldehydes as substrates.
The gene recombinants containing each of the mutations found in CTZ-resistant mutants were constructed using the wild-type sake yeast K1001.
It was shown that no specific bands appeared in the mutant; however, a specific band was exhibited using the wild-type strain, indicating that the ncRNA31 gene was completely knocked out in the mutant.
The extracellular enzyme activity accounted for 65.73% of the total activity, which indicated that the agarase can be extracellularly secreted using the wild-type signal peptide from Microbulbifer sp. Q7.
In the study, highly efficient repair of the mutant allele was achieved using the wild-type (WT) allele in heterozygous human zygotes through CRISPR/Cas9 (Ma et al., 2017).
Crystals of AP-aldolase are grown at two temperatures (4 °C and 18 °C), and the structure solved to 3.0 Å resolution, using the wild-type structure as the phasing model.
