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Simulations based on these numbers show that it requires nearly two times as much shotgun sequencing in different inbred strains separately to discover the same amount of SNPs that can be found using the wild rat shotgun sequencing approach.
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To estimate the number of SNPs to be discovered by the wild rat resequencing approach we performed computer simulations using the observed sample-specific polymorphism frequencies and the rat genome size of 2.48 Gbp as an input.
Since the wild rat sequences were quiet divergent from the inbred population we did not include these wild rat sequences in the neutrality tests.
And they used the wild dogs!
Use the Wild Goose Chase.
To get insight in the benefits of using wild rats in SNP discovery studies, we simulated larger scale experiments based on the results obtained in the experiments described above.
Using the rat genome database (rn3, Jun.
Dim red light can be used to calm wild rats and facilitate their handling and restraint in laboratory environments.
We used wild rats (Rattus fuscipes) to avoid potential effects of breeding on physiological phenotypes.
(I use the term "wild" here advisedly).
Finally, using a wild-type rat subcutaneous implantation model, we examined the extent of any immune reaction and confirmed that this matrix is well tolerated by the host.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com