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If you can't find it, I'd suggest using the whole leaf frozen variety instead.
Protein samples were isolated from the same samples that were used as for RNA extraction, or, for additional samples, the samples were collected in the same way, from the third leaf from the top, and using the whole leaf.
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Using the whole beast?
Following microcentrifugation, the supernatant was taken for use as the whole leaf extract.
From the AF proteins, 66% were predicted with a signal peptide sequence using the TargetP software [ 28], compared to 5% for the whole leaf proteins.
In addition the nitrogen isotopic composition of the whole leaf and the acid hydrolysed fraction of the leaf could be used in a similar manner.
In summary, the carbon isotopic signature of the whole leaf, free lipids, water-soluble extract, and the residual leaf can be used to distinguish dwarf and tall red mangrove leaves.
Cut as you need them for use, trimming right down to the base.When cutting parsley, always remove the whole leaf, together with the leaf stalk, nipping it back to where it joins the clump.
Plants were grown under identical conditions as those used for the whole-leaf glasshouse elicitation, but came from the 30th inbred generation of seeds originally collected at Lytle Ranch Preserve.
On the whole, Leaf is much less optimistic than Mukherjee.
Leaf wilt was observed at 10 days post-inoculation, and the whole leaf was scorched by approximately 3 wpi.
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