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We found that only one kidney could be evaluated using the whole kidney area.
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In our experiment, we used the whole kidney as a tissue source for RNA preparation.
Additionally the mouse model expression studies used the whole kidney as opposed to our use of renal medulla in this study.
Using the whole beast?
The contribution from activity in the remainder of the body to the absorbed dose to the kidney was estimated using the whole-body cumulated activity (assumed to be uniformly distributed and excluding the renal cumulated activity) and whole-body-to-kidney S values for 177Lu from RADAR/OLINDA [31].
Again, one important difference between the two studies is the source of renal mitochondria used: we used whole kidney mitochondria, while Katyare and Satav used cortical mitochondria as discussed above.
Some of the authors of previous studies have used a so-called a small VOI method [7], where a small VOI is used to sample activity within the whole kidney.
In order to compare the FM study in kidney to the two other methods used, the mean fluorescence intensity of the whole kidney, including the glomerulus (excretory compartment) and parenchyma (collecting compartment), was plotted against time in Figure 3C.
Real-time PCR data of BMP-7 expression in the whole kidney were evaluated using the Kruskal-Wallis test followed by the Mann-Whitney-U test for comparison.
Quantification was done manually by tracing the whole kidney cortex for the radioactivity density (counts/mm2).
Cells were disrupted from the whole kidney tissue by pipetting the suspension repeatedly.
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