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The JKM was processed using the wet fractionation method of Betancur-Ancona et al. [ 8].
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NMR spectra were acquired using the 1D wet pulse program (WET) included with the standard Topspin release version 1.3 [ 27].
Mitochondrial and cytoplasmic fractions were isolated using the Mitochondria/Cytosol Fractionation Kit # K256-100 (Biovision).
After exposure, cells were subjected to cytoplasmic and nuclear fractionation analysis using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, Beijing, China) according to the manufacturer's instructions.
Overall, these results fully agreed with those obtained using the alternative fractionation method.
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