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By using the waste droplet to increase pressure in the extraction zone, sampling buildup was greatly reduced and carryover between samples was less than 2%.
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The high-speed recordings were analyzed using the droplet morphometry and velocimetry software to obtain droplet size and droplet frequency statistics.
The fluorescence of each thermal cycled droplet was measured using the QX100 droplet reader (Bio-Rad).
Droplets were analysed using the QX100 droplet reader according to the manufacturer's instructions.
Following normal thermal cycling, droplets were individually scanned using the QX100™ Droplet Digital™ PCR system (Bio-Rad).
To create tumor spheroids for the externally-applied compression experiments, we used the hanging droplet method.
Using the Thunder Badge just wastes BP.
In this work, droplet jumping induced by multi-droplet coalescence was simulated using the volume of fluid (VOF) method with various coalesced droplet number and locations considered.
The droplet volume measured using the DG8 cartridge is slightly smaller than the volume generated using the beta-version of the droplet generator [ 29] demonstrating the importance of re-measuring the droplet volume when new generators or cartridges are released.
Using the NIH image analysis program all individual lipid droplets were identified, counted and encircled to compute lipid droplet area and derived morphometric parameters.
Droplets used for optical imaging to determine droplet volume were prepared using the same reagents as used for ddPCR estimation of Lambda DNA concentration.
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