Equivalent band areas across samples were selected using the volume rectangle tool.
Bands were selected using the Volume Rectangle Tool, and the 'Adjusted Volume Intensity mm value' was used as an arbitrary unit of intensity.
Proteins were quantified, using the Volume Rect Tool (Quantity One, Biorad), and the relative volume of the background was set to 1. Lymphoma/leukemia and EOC cell lines were fixed in 4% paraformaldehyde (PFA Thermo Scientificic) for 10 minutes.
Western blotting data was quantified using the volume analysis tool of QuantityOne 4.6.3 1D Analysis Software (Bio-Rad, Hercules, CA, USA), with Adjusted Volume (Intensity*mm2) as quantitative parameter, and the values expressed as means ± standard errors from two independent sets of data.
H-MR imaging was used to plan the C slice selective spectroscopy geometry and obtain reference images to calculate the tumour volume that was included within the C-MR spectroscopy slice (measured using the volume rendering tool in OsiriX (Pixmeo Sarl, Bernex, Switzerland)).
The volume of the nanoparticle and chromate was calculated using the Volume & Surface tool of Materials Studio 8, and this volume was subtracted from the volume of the periodic box in order to calculate the volume available for H2O molecules of solvation to be placed in the cell.
PAT volume was measured in ml using the volume analysis software tool of the Siemens Leonardo Circulation workstation.
Pericardial fat assessment was performed as described previously using the Volume analysis software tool of the Siemens Leonardo Circulation workstation [ 16].
Plots of parameter estimates are extracted using the volume of interest eigen variates tool in SPM5 with a sphere of 6 mm around the peak voxel identified from the significant contrast of interest in the key areas described in our a priori hypotheses namely, the ventral striatum and orbitofrontal cortex.
In particular, we used the "Volume Rect Tool" function to acquire pixel intensity data for each band.
