TUNEL-positive cells were counted in five different visual fields using the visualizing software Cell F (Olympus) and expressed as the percentage of positive cells.
Bober [ 77] showed the possibility of usefulness of the visualizing reagents such as Janus blue, methylene violet, gentian violet, methyl green, cresol red, Rhodamine B, malachite green, methylene blue, eosin yellow, and metanil yellow in the detection of selected quinolones and fluoroquinolones.
The visualizing reagents have the special significance to detect separated compounds on thin layers.
The best results of detection of stigmasterol and β-sitosterol were obtained by spraying the visualizing reagents (Tables 4 and 5) [ 47].
In view of the detection mechanism of the compound, the visualizing reagents can be sorted as follows: conservative reagents, which do not destroy separated substances destructive reagents, which destroy or change the structure of separated substances.
Visualizing effects of detection of cholesterol derivative depend on several factors, including the chemical structure of the visualizing reagents, the structure of the detected substance, and the type of chromatographic sorbent.
Dipping of chromatographic plates into solutions of the visualizing reagents leads to chromatographic spots of the fatty acids with better contrast than the spots obtained by direct spraying of the plates.
It was shown that visualizing effect depends on the chemical structure of the visualizing reagent, the structure of the substance detected, and the chromatographic adsorbent applied.
The visualizing effect depends on the chemical structure of the visualizing reagent, the structure of the substance detected, and the chromatographic adsorbent applied.
