Your English writing platform
Free sign upSuggestions(1)
Exact(1)
The results confirm the genotyping results using the variant specific analysis.
Similar(59)
Using the variant-specific PCR assay, the expression profiles of the seven v-rDNAs were examined in corneal epithelium of basonuclin-null and control mice (Fig. 4).
We used the variant-specific PCR assay to assess the expression level of each v-rDNA (Fig. 6A).
This result was expected, as E1 was expressed under the control of the CaMV 35S constitutive promoter and was targeted to the apoplast using the soybean variant-specific protein VSPβ secretion signal peptide.
We use the variant index v = 0 to refer to the master process and v > 0 to refer to context-specific process variants.
All splice variants were verified by PCR using splice variant specific primers.
qRT-PCR was performed using transcript variant specific primer pairs for both AdCa and SCC.
Using splice variant specific primer pairs we were able to detect EP3 subtypes 3, 5, 7 and 8 and to exclude the expression of EP3 subtypes 4 and 6 in HT-29 cells.
Relative copy numbers of V1, V2, and V3 were determined using variant specific selective primers.
Amplifications of the fragments corresponding to the Lpx-B1 transcript variants were performed using the same specific primer pairs (Table 1) and the amplification conditions used for amplification of the corresponding gene fragments, with the notable exception that 30 cycles were used to ensure semi-quantitative evaluation of the abundance of the mRNA sequences analysed.
Then, the specific activities of the four variants were assayed by using the specific substrate GD4K-βNA and the non-specific substrates Boc-E(OBzl -AR-MCA and Z-FR-MCA.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com