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By using the validated target organ search in miRWalk [ 25], 11 out of the 30 differentially expressed microRNAs were found to target both blood and lungs.
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A parametric study was conducted using the validated model.
Simulations are also carried out using the validated model.
Disease activity was assessed using the validated DAS28 (ESR).
For example the cel-let7 results show that an average of 14869 regions had a score that was at least as high as the left out sequence using SBM whereas an average of 92332 regions scored at least as high as the validated target using miRanda.
This difference was more pronounced in the dme-miR-7 results where an average of 273 sequences scored equal to or better than the left out sequences and an average of 8868 sequences scored at least as high as the validated target using miRanda.
The SBM method returned an average of 745 sequences scoring equal to or better than the left out sequence for dme-miR-4 in comparison to an average of 11488 sequences that scored at least as high as the validated target using miRanda.
The validated target section contains experimentally validated CDS- and 5′UTR-located miRNA targets.
Subsequently, we searched the validated target sites from our predicted targets, and found that MtiBase recaptured nearly 10 validated sites (Supplementary Table S3).
We used the validated German version [ 29].
We used the validated methodology [ 1].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com