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Comparisons between groups were performed using the unpaired samples Student's t test.
Baseline differences were tested using the unpaired samples t test.
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The differences between groups were tested using the unpaired (independent samples) or paired samples (for paired data) Student's t-test or one-way analysis of variance (ANOVA) analysis followed by Tukey's post hoc test for multiple comparisons as indicated.
To compare the different genotypes, p-values were calculated using the unpaired two-sample Student t-test assuming equal variances.
Comparisons between sheared and unsheared groups were made using the unpaired two-sample t-test, while comparisons in GFP fluorescence intensity measurements between time points were made using the paired two-sample t-test.
A volcano plot was used to identify differentially expressed genes using the unpaired two-sample Student's t test, assuming significance for P values ≤ 0.05, and fold changes ≥1.5.
The proportion of HUVECs in each phase of the cell cycle was compared between proliferating and quiescent samples using the unpaired t-test.
For continuous variables, the comparisons were carried out using the unpaired t-test for two independent samples, the Mann-Whitney U test for non-normal data and paired t-test for matched samples.
Unpaired, non-parametric samples were tested using the Mann–Whitney U test and unpaired, normally distributed samples were compared using the unpaired Studentʼns t-test.
Statistical analysis was performed using the Prism software, all samples were analyzed using the unpaired and 2-tail T-test.
The difference between the means of two independent samples was evaluated using the unpaired Student's t-test.
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