Sentence examples for using the univec database from inspiring English sources

Green, unpublished) was then applied to screen for adaptor sequences and other artifacts of the pyrosequencing procedure and also vector sequences using the UniVec database (www.ncbi.nlm.nih.gov/VecScreen/UniVec.html).

For GroupA, SeqClean was used with the default parameters to detect contaminant sequences using the UniVec database [ 54], because dbEST often contains such contaminants [ 55].

Electropherograms were analyzed with Phred [ 56, 57] to assign a quality score and with a perl script using the UniVec Database [ 58] to identify any vector and adaptors sequences.

Basecalling, quality filtering and trimming was carried out with phred v0.071220.b using the default quality cutoff settings, after which crossmatch v1.090518 was used to screen out remaining vectors using the UniVec database [ 74].

Generated ESTs were trimmed via the Consed suite of programs [ 51], including the 'Cross-Match' algorithm [ 52] using the UniVec database (http://www.ncbi.nlm.nih.gov/VecScreen/UniVec.html) to remove vector sequences and low quality reads.

Green, unpublished) was then applied to screen for adaptor sequences and other artifacts of the pyrosequencing procedure and also vector sequences using the UniVec database http://www.ncbi.nlm.nih.gov/VecScreen/UniVec.html.

Prior to assembly, regions of low-complexity were masked using RepeatBeater (BIOMAX Informatics, Martinsried, Germany) and vector remnants were removed using CrossMatch with the UniVec database [ 87] modified to include the specific polylinkers we used in the cloning process.

The sequences were further screened for adapter and vector contamination using NCBI VecScreen and the UniVec database.

Sequences were trimmed for low quality regions, vector contamination, and poly-A tails using SeqClean and the UniVec database.

First, cDNA sequences were trimmed using Seqclean (http://www.tigr.org/) and the Univec database (http://www.ncbi.nlm.nih.gov/VecScreen/UniVec.html).

Then, vector sequences were trimmed from the raw Sanger reads using cross_match (Cross_match http://www.phrap.org/) against the UniVec database.