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We searched the raw data obtained from the LC-MS/MS analysis using the Uniprot website.
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Protein links are given to Bio2RDF [2] using the UniProt identifier [26].
InterPro [25] domain annotations were retrieved for all protein targets using the Uniprot database [26].
Using the Uniprot database (http://www.uniprot.org), we identified the number of TM helices present in TMD dimers, together with the pI values of the SBPs in 35 importers (Table S1).
Sequence alignment of the PCNA sequences was performed using the Uniprot Blast tool [32].
The functions of the identified proteins were inferred using the UniProt database (http://www.uniprot.org).org
For dN/dS analysis of individual PPR domains, repeats were defined using the Uniprot model for each gene http://www.uniprot.org/uniprot.org/uniprot
The Uniprot-trEMBL database was annotated with GO terms using the Uniprot annotation file [ 52] (downloaded 20 November 2009).
The procedures for filtering the raw data from UniProt database are described as follows: First, the reviewed sequences in the UniProt database, which are annotated with subcellular location "mitochondrion," were retrieved using the UniProt online query and retrieval system.
The homologies were determined by BlastP interrogations against UniProtKB/Swiss-Prot protein database (January 2010) using the UniProt on-line tool http://www.uniprot.org, setting parameters to default.
Function, tissue specificity, and involvement in disease of the identified plasma proteins were determined using the UniProt database (http://www.ebi.uniprot.org/).org/
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