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However, if abnormal miRNA expression profiles are collected using the typical methods of analysis of canonical miRNA or the sum of all isomiRs, the difference in fold change values may affect the collection of deregulated miRNA species and may require further experimental validation.
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In order to verify the modulation of autophagy using the typical induction method by starvation and the inhibition method using the pharmacological inhibitor 3-Methyl Adenine (3MA) in the human PBMCs system used in this study, Western blotting of the two LC3 fractions was performed.
The recovery yield of microcrystalline cellulose was 97.75% (w/w), in agreement with the result obtained using the typical AACC method, demonstrating the reliability of the improved method.
A 250 g portion of the powdered leaves was extracted using the typical acid/base extraction method for alkaloids, as reported by [13], but the volumes of solvents were adjusted.
According to Shapiro, "We will not be using the typical on-sale method.
Mitochondrial RNA was extracted from calli using the typical phenol/chloroform RNA isolation method.
"The user was now not using any of the typical methods to access MITnet to avoid all usual methods of being disabled," concluded Mike Halsall, a senior security analyst at M.I.T., referring to the university's computer network.
Experimental determination of the MMP can be performed using the following typical methods.
The proposed method is more efficient than the typical methods used in the comparison.
This section describes the typical methods used to ultimately obtain individual ventricular myocytes.
Focus groups (or Mega Focus groups) are among the typical methods that can be used to support a participatory process [25].
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