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Paraffin-embedded sections from leg muscle were examined for DNA strand breaks using the TUNEL technique (In Situ Cell Death Kit, Roche Molecular Biochemicals) as described by the manufacturer.
Apoptotic cells were detected using the TUNEL technique.
Myocardial apoptosis was quantified by detection of DNA fragmentation using the TUNEL technique.
We detected apoptosis using the TUNEL technique (In Situ Cell Death Detection Kit; Roche Applied Science, Mannheim, Germany) according to manufacturer's instructions.
The apoptotic cells were identified as previously described (Ramos et al. 2002) using the TUNEL technique (ApopTag, Serologicals Corporation, Norcross, GA, USA).
However, to understand the extent of cell death in this model, as well as whether the MMP-3 inhibitor protects against cell death, further investigations using the TUNEL technique are required.
Similar(50)
To determine precisely which ovarian cell types and stages had alterations in nucleosomal fragmentation, we used the TUNEL technique on egg chambers.
We evaluated synovial apoptosis using the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling) technique and staining for the active form of caspase-3 in 2% formaldehyde-fixed sections as previously described [ 17].
Apoptosis was confirmed using the TUNEL assay for each stimulus.
Apoptotic cells were detected in situ using the TUNEL assay (TUNEL POD kit, Roche Molec Biochemic, Mannheim, Germany).
The presence of apoptotic cells within the tumor sections was evaluated by the TUNEL technique using the DeadEnd Fluorometric TUNEL System (Promega, Madison, WI, USA) following the manufacturer's protocol.
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