For example, we have recently shown, using the triple transgenic (3xTg-AD) mouse model of the disease, that stress hormones [8], or nicotine [9] intake increase the severity of AD pathologies, whereas increased dietary intake of omega-3 fatty acids [10], nicotinamide [11], or increased cognitive stimulation [12], can protect against formation of AD pathologies.
We used the hTau transgenic mouse, which overexpresses wild type human tau with a native promoter [ 125], and, to model AD, we used the triple transgenic rat (Swedish mutation in APP and mutation in presenilin-1 and presenilin-2) [ 126].
Using the triple-transgenic mouse model of Alzheimer's disease (3×Tg-AD), which develops amyloid and tau pathologies in a pattern reminiscent of human Alzheimer's disease, we initiated chronic intrahippocampal expression of IFNγ through delivery of a serotype-1 recombinant adeno-associated virus vector (rAAV1-IFNγ).
Similar results were obtained using the triple-transgenic mouse model of AD (3xTg-AD), which presents increased Aβ levels and develops tau and synaptic pathology, hallmark features of AD (Oddo et al, 2003).
120, 121 In a study from our lab using the triple-transgenic mouse model of AD (3xTgAD mice), the mice were maintained for one year (beginning at five months of age) on either ad libitum (control), 40% caloric restriction (CR), or IER (alternate-day fasting) diets.
Every procedure was performed using the triple freeze/thaw protocol.
The triple transgenic mice harboring APPswe, tauP310L, and PS-1M146V (3xTg-AD) [36] have the advantage of the age-dependent development of multiple pathological events relating to AD.
Green and colleagues demonstrated decreased presenilin 1 (γ-secretase) in the triple transgenic mice as a result of DHA supplementation.
An inconsistency was observed for serotonin levels and serotonin release with significant changes only in the triple transgenic model.
Results The authors addressed this issue by characterising circadian patterns of body temperature and activity in an animal model of AD, the triple transgenic AD mouse (3xTgAD).
Moreover, we show for the first time that APP 110 kDa isoform is reduced in the triple transgenic AD mouse model.
