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The clean reads of the three libraries were de novo assembled using the Trinity Program (V 2.2.0) with default parameters (Grabherr et al. 2011).
The transcriptome reads of P. monodon were assembled into 182,648 unigenes through using the Trinity program [ 82].
The transcriptome sequences were assembled using the Trinity program.
De novo assembly of the clean reads was performed using the Trinity program [ 55].
High-quality clean reads were assembled de novo into contigs using the Trinity program.
All the reads were then assembled into 66,815 unigenes using the Trinity program [ 82].
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We used the Trinity program suite to assemble all 13 tissue libraries including female bursa copulatrix and bursal gland libraries, as well as the two thorax libraries into a single assembly [ 65].
The de novo assembly of the quality processed reads using the Trinity assembly program resulted in 209,654 transcript isoforms.
De novo assembly of the processed reads into transcripts was carried out using the Trinity assembly program release 2013-02-25 (http://trinityrnaseq.sourceforge.net) [ 21] using default parameters.
Using the Trinity assembly program, we generated a total of 62,722 unigenes, the average length was 960 bp, and the N50 length was 1,450 bp.
These were assembled into 115,739 putatively unique transcripts (PUTs) using the Trinity assembly program (Grabherr et al., 2011) and compared with the 4 September 2013 version of NCBI NR using Blastx (Altschul et al., 1997).
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