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We obtained four different transcriptomes from all tissues using the Trinity platform (Table 1, Additional file 3).
After removinging low-quality reads, all reliable reads were assembled using the Trinity platform to reconstruct a unigene library for the wheat resistance line N9134 [ 41], and DEG analysis was performed with the bioconductor package DESeq [ 42].
Because of deficiencies in the reference genome sequence, these reads were de novo assembled using the Trinity platform software, resulting in 186,632 unigenes with N50 length of 743 bp, of which 89,672 unigenes were annotated after Blast searches of the GenBank Nr, SwissProt, KEGG, COG and GO databases.
All reads for the RNA-Seq datasets derived from roots, floral buds, or seedlings of 10-day-old A. thaliana seedlings (Table 1) were used for de novo assembly using the Trinity platform [ 18].
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The clean reads of the three libraries were de novo assembled using the Trinity Program (V 2.2.0) with default parameters (Grabherr et al. 2011).
The transcriptome sequences were assembled using the Trinity program.
De novo assembly of the clean reads was performed using the Trinity program [ 55].
All the reads were then assembled into 66,815 unigenes using the Trinity program [ 82].
High-quality clean reads were assembled de novo into contigs using the Trinity program.
De novo assembly was performed using the Trinity program (release 20130225 [ 27]).
All six RNA-Seq data sets were pooled and assembled using the Trinity program (Fig. 2a).
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