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De novo assembly was performed using the Trinity method with default parameters [ 58].
Reads were assembled separately from each S. lycopersicoides library using the Trinity method [ 58].
These high-quality reads were de novo assembled using the Trinity method, and 22,941 unigenes with a mean length of 1,468 bp were obtained (Table 2).
Totally, 275,425,782 clean reads (including CK and DT samples) were used to assemble the transcriptome data using the Trinity method.
Using the Trinity method [ 20] the sequence reads were finally assembled into 40,587 non-redundant unigenes, spanning a total of 101 Mb of sequence with a GC content of 43.14%.
Using the Trinity method, 52,081,238 high-quality trimmed reads were assembled into a non-redundant set and 108,502 unigenes with an average length of 1,047 bp were generated.
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We obtained 108,502 assembled unigenes using the Trinity de novo assembly method and identified numerous potential cold sensing transcription factor genes and various key signal transduction components at the transcriptome level.
We got 54,870 assembled unigenes using the Trinity de novo assembly method, and a number of chilling regulated genes were identified, providing useful resources for gene mining to improve cold tolerance of plants.
The clean reads of the three libraries were de novo assembled using the Trinity Program (V 2.2.0) with default parameters (Grabherr et al. 2011).
The transcriptome sequences were assembled using the Trinity program.
Lactate secretion was determined using the Trinity Lactate Kit (Trinity Biotech, Jamestown, NY, USA) according to the manufacturer's protocol.
More suggestions(15)
using the Jadad method
using the Newton method
using the Framework method
using the trinity package
using the trinity algorithm
using the trinity tool
using the Trump method
using the trinity program
using the Wintrobe method
using the trinity setting
using the Delphi method
using the Fick method
using the trinity assembler
using the Tukey method
using the trinity platform
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