Sentence examples for using the transient transfection from inspiring English sources

These methods are more amenable to large-scale production of AAV vectors than methods using the transient transfection of mammalian cells.

Viral particles were produced using the transient transfection protocol[ 25].

However, significant results were not obtained using the transient transfection expression vectors in 293T cells, COS-1 cells, or HeLa cells (data not shown), likely because insufficient transfection efficiency.

We examined the effect of various PBDE congeners, OH-PBDE metabolites, and PBDE mixtures on TR-mediated transcription in fibroblast-derived CV-1 cells using the transient transfection-based reporter gene assay.

The incubation product was then tested in ERE-luciferase assays, using either the transient transfection system with MDA-MB-231 cells or the stably transfected ER-α positive BG1Luc4ER-α positive

293 T human embryonic kidney cells were used for the transient transfection studies with pcDNA3/ Mpl constructs of controls and patients used in the in vitro transcription/translation studies.

FuGene6 transfectin reagent (Promega) was used for the transient transfection of cell cultures.

HiPerfect Reagent (Qiagen) was used for the transient transfection.

Lipofectamine-LTX/Plus was used for the transient transfection of HUVECs.

Next day, Optifect-mediated transfection was used for the transient transfection assay according to the protocol provided by Invitrogen/Life Technologies , Inc

Targefect-Hepatocyte (Targeting systems) was used for the transient transfection assay of FOXO1 or CREB reporter (Qiagen) activity.