Sentence examples for using the transient expression from inspiring English sources

Next, we constructed a set of binary transformation vectors using the transient expression vectors as backbones.

Taken together the results encourage the investigation of chloroplast targeting for other malaria vaccine constructs using the transient expression system.

One such approach is 'Effectoromics', a high-throughput functional genomics approach that uses the transient expression of Avr effectors to probe plant germplasm to detect and characterize R-genes.

For instance, while a 50% coverage library would require ~380 individual transfections using the transient transposase expression strategy (~50 detectable transpositions per transfection) the same level could be obtained with far fewer transfections in parasites containing a stably expressed transposase.

However, significant results were not obtained using the transient transfection expression vectors in 293T cells, COS-1 cells, or HeLa cells (data not shown), likely because insufficient transfection efficiency.

Cotyledons from secondary SE were used for the TcBBM stable expression while cotyledons from primary SE were used for the transient expression experiment.

A hundred micrograms of plasmid DNA was used for the transient expression in 10 dishes (φ10 cm) of cultured human embryonic kidney (HEK) 293s cells.

Plant viral vectors can be used for the transient expression of mAb more rapidly than transgenic plants.

The plasmids used in the transient expression assay were constructed by ligating full-length LcSGR (GenBank: KP853137) and LcPPH (GenBank: KP853136) into the pEAQ-HT vector [ 71].

Recombinant A. tumefaciens carrying pTRAkc-GAP50-cTPH or pTRAkc-GAP50-ERH were used for the transient expression of PfGAP50 either as ER-retarded or as chloroplast targeted variant.

The constructs used for the transient expression of fluorescent markers for the Golgi [pBL 35S: Man49-mCherry)], peroxisomes [pBL 35S: mCherry-SKL)] and tonoplast [pBL 35S: γTIP-mCherry)] are derivatives of the organelle markers developed by Nelson et al. (2007).