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Further, in reply to Reviewer 2, we have noted that our previous work has shown that unexpected links to non-GSL phenotypes, such as flowering time, have been validated both with the transgenic and natural populations i.e. we could map circadian timing variation to GSL loci in an Arabidopsis RIL populations and then show the effect using the transgenic validation lines.
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This hypothesis could be tested using the transgenic animal model described above.
Then they can use the transgenic mice to figure out appropriate therapy for people.
2) The assay they are using for induced β-cell differentiation: Nowhere do the authors show a photo of validation using the original transgenics used in the screen.
(A) PCR analysis for putative Rs-cps transgenic plants using the primers RNAi-F/RNAi-R (lanes 1-15: different Rs-cps transgenic lines).
(B) PCR analysis for putative egfp transgenic plants using the primers eGFP-F/eGFP-R (lanes 1-11: diffegfptransgenicnsgenic lines).
Transgenics were generated using the I-SceI meganuclease system.
Bartram, D. A cross-validation of between country differences in personality using the OPQ32.
Called variants that had a minimum coverage of 10× in both the discovery and the validation sets were used for the validation.
The images from the subject with deuteranopia were used in the validation data set.
The FIB-1 GFP transgenic strain (COP262) was generated using a custom transgenic service (Knudra Transgenics, Salt Lake City, UT).
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