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Remove the WB using the transfer pipette and replace it with 1 mL 0.5% OsO4 solution for postfixation (staining) of about 1 h.
Multispecimen, flat, silicone embedding molds (TAAB Embedding mould type B E071). 60 °C degree thermostat. 1. Remove the WB using the transfer pipette and replace it with 1 mL 0.5% OsO4 solution for postfixation (staining) of about 1 h.
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At 1.5 dpc, plugged pseudo-pregnant female mice were anesthetized, CRE and AurkB/C mRNA injected ROSA 4-cell stage embryos were transferred into the oviduct using glass transfer pipette and the mice were nursed until vivification.
After 1-3 minutes for capture of the beads on to the surface of the slide (visible as clearing of the liquid around the magnet), the liquid was removed using a transfer pipette.
Gentle agitation was applied using a transfer pipette until the ventricles dissociated.
Seeds were suspended in 0.1% agar and 5 to 10 were sown using a transfer pipette in the rhizotron.
The lower airway portion of the induced sputum was selected using a transfer pipette and dispersed in dithiothreitol 0.1% at 20°C.
The water was then removed using a transfer pipette.
3. Add 100 μl/ml of 100% Acetic Acid in a fume hood.b 4. Re-suspend chitosan solution using a transfer pipette to wash off any chitosan material from the test tube walls. 5. Cover test tube with aluminium foil.
Following centrifugation, samples were placed on ice before being transferred, using a transfer pipette, to an appropriately labeled cryovial.
Plasma was removed using a transfer pipette and 0.5 ml aliquotted into 1.0 ml cryo tubes.
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