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We used Mapman10 web-tool to visualize the metabolome changes in Malus X domestica due to biotic stress by using the transcriptomic data of the 7 datasets.
Using the transcriptomic data, we compared our model predictions with measured lipid changes.
Using the transcriptomic data, we identified hemolymph peptides and proteins induced upon immune challenge by 2D-gelelectrophoresis combined with mass spectrometric analysis.
Step 2: FVA upper and lower bounds for the unregulated model are multiplied by the fold change value (c j ) obtained using the transcriptomic data.
To identify relevant transcriptional variation in the context of aroma compound production, PCA analysis and PLS1 and PLS2 models were constructed for the compounds in tables 2, 3, 4 using the transcriptomic data as X variables.
Where multiple protein products were identified from a single gene the correlation was calculated using the transcriptomic data for that gene versus proteomic data for each individual protein spot (i.e. multiple spots were not averaged or summed), because of the possibility that not all spots for any one gene product were detected.
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These data allow us to use the transcriptomic data from our previous study [4] to relate mRNA targets with the miRNAs identified in this study.
(a) Base quality using Phred scores for the transcriptomic data using F2-1 as a representative sample.
In general, the other models in addition to the use of the transcriptomic data alone regardless of the model, performed poorly in the generalizability assessment (Additional file 5: Figures S2 and Additional file 6: Figure S3).
In addition, existing MS/MS data sets, generated during our previous studies of C. fleckeri venom [ 6], were reanalyzed using the new transcriptomic data.
In addition to the in-gel digests performed as part of this study existing MS/MS data sets, generated during our previous studies of C. fleckeri venom [ 6], were reanalyzed using the new transcriptomic data.
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