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The game was also solved using the total system capacity as the payoff, as a zero-sum game.
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Total RNA was isolated from control or CHC siRNA transfected cells in a 6-well plate using the total RNA purification system (Invitrogen, Carlsbad, USA).
The diffusion coefficients and conductivity calculated using the total MSD of the system compare favorably with the corresponding experimental and ab initio values reported for most similar system as ours (see Table 1).
Razmi et al. (2010) considered a buyer supplier supply chain and compared the performance of the traditional and VMI system using the total inventory cost of the supply chain as the performance measure.
Electroporation was performed using the Gene Pulser Xcell total system (BioRad).
In this context, either a median system throughput of 46 Mbps/cell or a cell-edge user throughput of 2.6 Mbps was demonstrated to be feasible with the BB protocol using a total system bandwidth of 20 MHz.
Total RNA was extracted using the SV Total Isolation System Kit of Promega and subsequently cDNAs were synthesized with the RevertAid H Minus First Strand cDNA Synthesis Kit of Fermentas.
Cells were collected and total RNA was extracted using the RNeasy Total RNA system (Qiagen GmbH, Hilden, Germany) following the manufacturer's protocols.
As a result, the actual throughput rate of a cable modem is a function of total traffic on the branch; that is, as more subscribers use the system, total throughput per user is reduced.
Total cellular RNA was extracted using the SV total RNA Isolation System (Promega, Madison, WI, USA) and the first-strand DNA was synthesised using a cDNA Reverse Transcription kit (Applied Biosystems Japan, Tokyo, Japan) following the manufacturer's instructions.
We also measured the total time spent using the EPR system in 14 of the observed encounters.
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