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Using the tools implemented in BAMS, we organized the collated information, and further analyzed it statistically.
Similarity, identity based, networks were constructed using the tools implemented in the software B2N [ 15].
Samples were hierarchically clustered using the tools implemented in InforSense Workflow Builder http://www.inforsense.com.com
Phylogenetic trees of sequences encoding 16S rRNA were obtained using the tools implemented in the Ribosomal Database Project II [ 70] and compared to maximum likelihood phylogenetic trees of the corresponding EI sequences obtained as indicated above.
The prevalence of these genes was examined in the genomes of all BFo isolates, and to an initial list of 50 Erwinia, Dickeya, and Pantoea genomes using the tools implemented in BIGSdb (Jolley and Maiden 2010).
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The molecular surface including the electrostatic potential as well as the accessible surface areas were calculated using the corresponding tools implemented into the DeepView program.
These analyses were performed using the FASTQ tools implemented in the Galaxy platform [ 104- 106].
The resulting list of annotated genes was further analyzed for functional enrichment in Gene Onthology (GO) terms using the various tools implemented in the WEB-based Gene SeT AnaLysis Toolkit (WebGestalt, [ 75]).
All the simulations were analyzed using the tools for analysis implemented in GROMACS.
The length of the filaments was determined using the evaluation tool implemented in the aquisition software AxioVision (version 4.8.1, Zeiss).
Genes of interest were placed on the physical-genetic map of maize using the BLAST tool implemented by the Maize Genome Sequencing Project [42].
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