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The density of biochar surface charge was determined using the "titr_v3" programme.
The density of Pyrolox™ surface charge was determined using the "titr_v3" programme.
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New items in the repository are now synchronised hourly using the DSpace4 programme, RegisterDOI.
Secondary structures of tRNAs were accessed by using the RNA2 programme at the following website: http://www.genebee.msu.su/services/rna2_reduced.html.html
Using the N6software programme (QSR International Pty Ltd 2002), further coding, categorisation, and thematising analyses were performed.
The results of the MK and SK were combined using the CAP3 programme with default parameters [ 104].
To analyse the partial and alternative transcripts, the contigs were computationally fragmented to 100 bp tagged and mapped to the banana genome using the bowtie2 programme [ 50].
PCR was then performed using gene-specific DNA primer pairs that were designed using the Primer3 programme and synthesised by Eurofins MWG.
The results are presented as the mean ± SEM of three independent experiments in duplicate and analysis was performed using the SigmaPlot9.01 programme.
Using the MEGA5 programme (http://megasoftware.net/mega.php), multiple alignments were generated by ClustalW (www.clustal.org/omega), and phylogenetic trees were constructed by the neighbour-joining method.
To reduce the risk of identifying artificial SNPs arising from sequencing errors or misassembly of paralogous sequences, 454 reads were reassembled using the CAP3 programme under very stringent assembly criteria (overlap setting: 100 bp and 95% similarity).
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