and used for the tissue distribution and high-fat diet analyses; anti-mouse and anti-rat SIRT3 serum was also developed against the C-terminal regions of each respective protein (Covance), and the anti-mouse serum was validated for specificity using brown fat, cardiac tissue, and soleus muscle from SIRT3 knockout mice (Supplemental Fig. 1), then used for analyzing the exercise samples.
Using the tissue array, the expression and distribution of 17 angiogenic biomarkers were evaluated immunohistochemically.
There are several assumptions when making the Vss prediction using the rat tissue distribution data.
Introduction: Quantitative whole-body autoradiography (QWBA) is a technique used to determine the tissue distribution of radiolabeled compounds in laboratory animals.
Tumour-bearing mice were used to examine the tissue distribution of radioactivity after intravenous injection into the tail vein of 1 MBq of 18F-SIG343 or 18F-SIG353 in 100 μL of saline.
In the mouse, tissues were harvested representing the three VLCAD genotypes (+/+, +/−, and −/−) and were snap-frozen in liquid nitrogen, as previously described.[10] Frozen tissue stored at −70°C was used to assess the tissue distribution of TMEM135 in the heart, skeletal muscles, liver and brain of two-month-old mice.
Northern blotting was used to determine the tissue distribution and sizes of the mRNA encoding BTBD1 and BTBD2 (Fig. 2).
BPI was used to study the tissue distribution of bacteria in animals infected by the two routes and revealed startling differences.
Despite many technical obstacles that need to be overcome in this developing field [ 9], IMS has been successfully used to study the tissue distribution of lipids [ 10], proteins [ 11], peptides [ 12], and pharmaceuticals [ 13].
This protocol was also used to image the tissue distribution of [Zr(ox 4]4–; one mouse was injected with 9.3 MBq of [Zr(ox 4]4– in 200 μL ammonium acetate solution (0.1 M) via a cannulated tail vein and imaged as described above.
