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Bruchpilot (Brp) and GluRIIC puncta were manually counted at MN4b synapses on muscle 4 from confocal z-stacks and terminal area was calculated using the threshold function in Metamorph for the HRP channel as previously [36].
The background value for each image was set using the "threshold" function of the program to a level that would exclude all pixels in control tissues stained with secondary antibody alone.
The chloroplasts were tracked manually using the threshold function of ImageJ.
Positive labelling was selected form background using the threshold function of ImageJ with values constant between data sets.
In each image, the background value was set using the "threshold" function of the software to exclude all pixels surrounding the positive stained signals.
Using ImageJ, the RFP-LC3 and GFP-LC3 and lysotracker images were converted to black and white images using the threshold function, and the vesicles diameter for each lysosome was manually measured as previously described.
Similar(50)
Moreover, the population of mitochondria was too dense to use the threshold function.
Motivated by this fact, we follow [1], [25] in using the threshold linear function.
The percent area stained was quantified with ImageJ software using the threshold color function in RGB color space with pass thresholds set at red: 0 255, green: 0 104, and blue: 0 64.
Also using the threshold and measure functions, 10 fibrils were randomly selected, and their diameters were measured in each of six images per group.
A threshold was then chosen using the 'Auto threshold' function of ImageJ.
More suggestions(15)
using the cut off function
use the threshold function
using the standard function
using the ellip function
using the step function
using the lm function
using the histogram function
using the repair function
using the threshold value
using the search function
using the duleg function
using the share function
using the threshold p-value
using the image function
using the calibrate function
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