Sentence examples for using the threshold cycle from inspiring English sources

The standard curve had six points, and it was calculated using the threshold cycle method with the highest standard amplified being 2.3 ng of plasmid (~4.5*108 plasmid copies).

Fluorescent emission data were captured, and mRNA levels were quantified using the threshold cycle value.

The calibration curve was generated using the threshold cycle (Ct) of 8 serial dilutions of plasmid DNA template with known copy number.

Quantification of candidate gene expression in tissues (midgut, fat body and cuticle) was based on the Relative Standard Curve method [2] using the threshold cycle (Ct) values.

The expression levels of the target genes were calculated using the threshold cycle time (Ct), the first cycle number at which emitted fluorescence exceeds 10× the standard deviation (SD) of base-line emission as measured in the cycles of PCR.

The level of mRNA expression was calculated using the threshold cycle (Ct) value, the number of PCR cycles at which the fluorescence signal during the PCR reaches a fixed threshold.

Data were collected using instrument spectral compensations by the SDS 2.1 software (Applied Biosystems) and analysed using the threshold-cycle (Ct) relative-quantification method.

Expression profile in each specimen was assessed by using the comparative threshold cycle (2-ddCt) method.

Relative expression of target genes was calculated using the comparative threshold cycle method.

Relative quantification of mRNA was performed using the comparative threshold cycle method using GAPDH as an endogenous control.

The relative amounts of VASA were normalized against GAPDH using the comparative threshold cycle (CT) method [15].