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Samples collected from 2006 to 2009 (n=53) were genotyped using the TG method, whereas samples collected from 2010 to 2012 (n=97) were genotyped using the VS method.
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Carbonation depths were assessed using the TG-DSC method combining the results obtained from phenolphthalein indicator.
The thermal decomposition of cephalexine, cefadroxil and cefoperazone under non-isothermal conditions using the TG, respectively DSC methods, was studied.
Thus, we considered elucidating the explanations for this by investigating the serum TG profile using the VLDL-TG method developed by Okada et al. [8].
Liver and skeletal muscle TG content were measured using the Folch method for lipid extraction followed by spectrophotometric measurement of TG content (Thermo Electron, Louisville, CO) as previously described (Cummings et al., 2010b; Folch et al., 1957).
We estimated the TF activities based on the qPCR measured expression data from bmp8b TG vs. WT mice using the "msviper" method from the "viper" R package (https://doi.org/10.1038/ng.3593).3593
Standardised measurements of TC, TG, FBG and HCY will be performed using the enzymatic method.
Hepatic TG was determined from liver tissue extraction using the chloroform/methanol method (2 : 1) described previously [ 16].
Estimates using the methods described here (see Methods Section below) predict 4.9 Tg C (from CO2) and 5.3 Tg C (from CO2 and CO) from the Biscuit fire (in Oregon only).
mRNA expression levels of the target genes (TG) were quantified using the ΔΔCT-method with multiple housekeepers as previously described [ 15, 16].
Lipid was extracted from livers using the method of Folch et al. (1957), and hepatic and plasma triglyceride (TG) were measured by the colorimetric method using TG-IE kits (Wako).
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