The proportion of live cells was determined using the tetrazolium-based colorimetric cell proliferation assay (MTS, Promega) by reading the absorbance at 560 nm.
The viable cell growth after incubation with extract, fractions and isolated compounds was determined using the tetrazolium-based colorimetric MTT assay described by Mosmann (1983) [ 13].
Viability of cells was determined using the tetrazolium-based colorimetric MTT assay (3-5-dimethyl thiazol-2-yl-2, 5-diphenyl tetrazolium bromide) described by Mosmann [ 39].
Viability of cells was determined using the tetrazolium-based colorimetric MTT assay (3-5-dimethyl thiazol-2-yl-2, 5-diphenyl tetrazolium bromide) described by [ 21].
The cytotoxicity of V γ9V δ2 T cells against tumour targets was determined using the tetrazolium-based (MTS) assay referred to as CellTiter 96 (Promega, Madison, WI, USA) as described previously (Mattarollo et al, 2007).
The cytotoxicity of Cy was evaluated as described by Stevignyetal (2002) using the tetrazolium salt MTT (3 4,5-dimethylthiazol-2-yl -2,5diphenyltetra-zolium bromide (Sigma) colorimetric method based on the cleavage of the reagent by dehydrogenases in viable cells (Mosmann, 1983 4,5-dimethylthiazol-2-yl -2,5diphenyltetra-zolium
Cell viability was quantified after 24, 48, and 96 h of culture growth using the tetrazolium salt MTT assay.
Effects of EBP1 and IPA-3 on tamoxifen sensitivity were measured using a tetrazolium based cell viability assay.
The effect of kefir on the proliferation of leukemia cells was measured using two kits: one based on an MTT method that measures the conversion of tetrazolium salt into a formazan product, and another one that uses the tetrazolium salt WST-1 which, when in contact with metabolically active cells, becomes cleaved to formazan by mitochondrial dehydrogenases.
Cell viability was determined using the standard tetrazolium-based colorimetric assay [ 23].
