Sentence examples for using the test primers from inspiring English sources

The correct integration was determined by PCR using the test primers as described below.

Genomic DNA of each clone was extracted, and the correct integration was firstly verified by PCR using the test primers.

The mutant was verified by PCR using the test primers KOnudF-VF/KOnudF-VR.

DNA from the same 94 samples, which were previously tested by using the PLF/UNR/VIR primers, was tested again with the three assays listed above.

Using the RELATE test in PRIMER, we tested the Spearman rank correlation between the faunal similarity matrices as faunal abundance and percentage of groups, and model similarity matrix based on three different types of substratum, i.e. that faunal similarities are related to depth differences and substratum types among transects.

Essentially, we evaluated the methylation levels of sequences obtained from bisulfite conversion of the tested primers using serial mixtures of DNA samples obtained from hippocampal tissues of two adult male mice, one from a saline-control litter and the other from a cocaine-treatment litter.

The application of those two techniques revealed that all but nine of the samples that had initially tested positive by the PCR test using the Uhlmann primers were, in fact, not measles virus".

We used the 2−ΔΔCt method with the tested primers to calculate relative expression levels of the transcripts; the efficiencies for the various real-time PCRs were determined to be close to 100%.

This software mimics the PCR amplification with the tested primers and using as template the DNA sequences present in a collection of bacterial DNA sequences of non-GM bacterial genomes of 248 strains, representing 122 species belonging to 72 genera, retrieved from the NCBI public database [ 34].

However, "the D'Souza group ... subjected those apparently positive samples to additional testing techniques in order to determine whether the PCR testing using the Uhlmann primers was truly identifying measles virus and only measles virus..

Transduction of the bldD::apr allele was confirmed by PCR using test primers listed in Table S4, and the strain was named SV74.