Exact(1)
Target prediction information for human and mouse were directly downloaded from the TargetScan homepage (file Conserved_ Site_Context_ Scores.txt, release #62) and calculated for rhesus monkey, opossum and chicken miRNAs using the TargetScan tool kit, applied to all miRNAs and the 3'UTRs from these organisms.
Similar(59)
To identify post-transcriptional regulators of LXRα, we performed in silico analysis of LXRα mRNA sequence using the TargetScan microRNA prediction tool.
We first obtained miRNA lists containing broadly conserved miRNA families, conserved miRNA families and poorly conserved miRNA families, and then retrieved web pages of conserved targets for each miRNA family using the TargetScan on-line search tool.
To identify the functions of miR-183 in HCC cells, we analyzed putative target genes by using the TargetScan http://www.targetscan.org bioinformatics tools [ 18]. miR-183 maybe play the role of oncogene, considering it is up-regulated in HCC.
The subsequent analysis of miRNA targets prediction and target gene functional annotation was performed using the TargetScan software (http://www.targetscan.org/) and the DAVID gene annotation tool (http://david.abcc.ncifcrf.gov/), respectively.
Using the TargetScan program, a custom set of Perl codes were downloaded from the TargetScan database (Release 5.1, http://www.targetscan.org/).org/
We next performed target prediction analysis using the TargetScan software package [ 51].
First, we screened miR-218-targeted genes using the TargetScan database and identified 2940 genes.
We identified putative miRNA targets using the TargetScan Fly [ 52] and MinoTar data bases [ 53].
We used the TargetScan prediction tool [30] to rank all genes according to their miR-29c target prediction scores.
To identify potential miR-181a putative targets, we used the TargetScan 6.2 database.
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