Sentence examples for using the taqman technology from inspiring English sources

The qPCR reaction was optimized by using the TaqMan technology and an internal positive control was included to ensure the absence of PCR inhibitors.

Amplification of viral RNA was achieved by using the TaqMan technology (Fig. 1D).

Following extraction of total genomic nucleic acids, all viral RNA forms were quantified by real-time RT-PCR using the TaqMan technology (see Table S1 for primers and probes sequences).

Real-time PCR was performed on a 7500 Real-Time PCR System apparatus (Applied Biosystems) using the TaqMan technology (QuantiTect Probe PCR Master Mix, Qiagen).

Relative quantification of mRNAs encoding PKR, MxA and the 40 kD isoform of 2′5′OAS (2′5′OAS p40) was performed using the TaqMan technology.

Real-time PCR for quantification of CR-1 mRNA was carried out with a 7900HT ABI PRISM Instrument (Applied Biosystems, Milan, Italy), using the Taqman technology (Applied Biosystems) as previously described (Normanno et al, 2004b).

Real-time qRT PCR assays for CEA, CEACAM6, CEACAM1-S, CEACAM1-L, CEACAM7-1, CEACAMUC2, MMP7, MMP7 and CK20 mRNAs were constructed in the laboratory using the TaqMan EZ technology (Applied Biosystems, Foster City, CA, USA).

Consequently, a real time qRT PCR assay with RNA copy standard detecting all known splice-forms of KLK6 mRNA was constructed by using the Taqman EZ technology (Applied Biosystems) as described (Fahlgren et al, 2003; Ohlsson et al, 2012).

Genotyping was performed using TaqMan Pre-Designed SNP Genotyping Assays® (Applied Biosystems, Foster City, CA, USA) on the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using the TaqMan Allelic Discrimination technology [25].

QM-MSP was performed in triplicate to detect and quantify simultaneously three methylated markers in control and DNA sample using the TaqMan MBG probes technology (Life Technologies).

miRNAs were further profiled using the Taqman Low Density Array technology.