Sentence examples for using the taqman quantitative from inspiring English sources
To investigate the validity of array data, expression levels of the differentially expressed genes were measured using the TaqMan quantitative RT-PCR assay.
Samples were tested for the presence of various arboviral RNA genomes by using the TaqMan quantitative reverse transcription PCR (qRT-PCR) technology and specific primers and probes (protocols available upon request to the corresponding author).
MicroRNA expression was measured using the TaqMan microRNA quantitative PCR kit (Applied Biosystems) as previously described [12] and normalized with the endogenous control, RNU48 (purchased from Applied Biosystems).
Expression of mRNA was analysed using the TaqMan real time quantitative PCR system (RT-qPCR; Applied Biosystems).
One-step real time RT-PCR methods using the TaqMan probe are described for quantitative detection of RSV in rice tissues and in Laodelphax striatellus Fallen, the small brown planthopper (SBPH).
A real-time PCR method using the TaqMan probe is described for quantitative detection of WDV in wheat tissues and in leafhopper (Psammotettix alienus Dahlb).. Primers and probes for specific detection of WDV were designed within the conserved region of the coat protein (CP) gene sequence.
Analyses were performed at the Harvard School of Public Health using the TaqMan 5′ nuclease real-time quantitative PCR assay.
96 genes were tested by quantitative PCR, using the TaqMan low density micro fluidic card (Applied Biosystems, USA).
All of the insertions/deletions within each gene and in intergenic regions were analyzed by real-time genomic quantitative PCR using the TaqMan method (Applied Biosystems).
The heparinase-treated materials were subjected to quantitative PCR using the TaqMan universal PCR master mix chemistry and the species-specific DNA primers and probes.
To verify estimated CNV regions, we carried out quantitative PCR using the TaqMan Copy Number Assay (Life Technologies, Foster City, USA) according to the manufacturer's protocols.
