Sentence examples similar to using the taqman procedure from inspiring English sources

The quantitative real time PCR was performed according to an established procedure using the TaqMan system [ 34] (PCR, ABI 7700, PE Applied Biosystems, Rotkreuz, Switzerland).

In order to increase the sensitivity of the TLDA, a pre-amplification was performed after the RT procedure using the TaqMan PreAmp Mastermix and the Megaplex PreAmp Primer Pools A + B (Applied Biosystems).

Quantification of miRNAs from tissue samples was carried out by a two-step procedure using the Taqman LDA Human microRNA Panel v3.0 (Micro fluidic card, Applied Biosystems, Foster City, CA) according to the manufacturer's protocols.

PCR reactions were performed on a LightCycler 480 PCR system (Roche) using the TaqMan Universal Master Mix II Applied Biosystemss).

cDNA was synthesized using the TaqMan MicroRNA Assays Kit and High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

A first step was to confirm mature let-7 levels in the various samples using the Taqman quantitative PCR assay.

Sequences of pre-made primers and in-house designed primers are shown in supplementary table 3. TaqMan gene expression assay for PKM2 and Cldn5 were used in combination with TaqMan Fast Gene Expression Master Mix Applied Biosciencess) and expression was normalized to endogenous GAPDH also using the Taqman gene expression assay.

Genotypes were determined using the Taqman allelic discrimination assay.

To quantify the miR156 and miR172 expression, PCR was performed using the TaqMan Fast Universal PCR Master Mix (Applied Biosystem).

Deoxyribonucleic acid was extracted from paraffin-embedded tissue sections, and genotype was determined using the Taqman method.

We genotyped SNPs using the TaqMan assay.