Sentence examples for using the taqman miRNA from inspiring English sources
Reverse transcription of miRNA was performed on 11.35ng total RNA using the TaqMan miRNA Reverse Transcription Kit (Applied Biosystems), and pooled miRNA-specific primers.
cDNA was synthesized from 5 ng of total RNA using the Taqman miRNA reverse transcription kit (Applied Biosystems, Foster City, CA), and the expression levels of miR-96 were quantified using miRNA-specific TaqMan MiRNA Assay Kit (Applied Biosystems).
qRT-PCR was then performed in triplicate using a Rotorgene 6000 (QIAGEN) with the PCR products being amplified from the cDNA samples using the Taqman miRNA assay together with the Taqman Universal PCR Master Mix Applied Biosystemss).
Five µl of total RNA was reverse transcribed using the TaqMan miRNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA) and miRNA-specific stem-loop primers (part of TaqMan miRNA assay kit; Applied Biosystems).
cDNA was synthesized using the Taqman miRNA RT Kit (Applied Biosystems).
RT-qPCR was carried out using the TaqMan MiRNA Assay (Applied Biosystems, Foster City, CA) according to the manufacturer's protocol.
These discrepancies may be technical since we used home-made arrays for the identification of miRNA expression while Lee and co-workers used the TaqMan miRNA assay [18].
We used the TaqMan miRNA array to obtain the 365 miRNA expression profiles from the parent and gemcitabine-resistant pancreatic cancer cell lines.
Expression profiles for the 365 mature miRNAs were obtained by quantitative polymerase chain reaction (qPCR) using the TaqMan Array Human MiRNA (TLDA) or TaqMan MiRNA Assays (Applied Biosystems ABII) on an ABI Prism 7900HT according to the manufacturer's instructions.
The expression levels of unprocessed transcript of MIR17HG (pri-MIR17HG) were examined using the TaqMan Pri-miRNA assay (Life Technologies) as described previously [ 16].
Primary MCT miRNA expression profiling was performed at the OSU Nucleic Acid Shared Resource using the TaqMan Array Human miRNA Panel (Human A Cards, v.2, Applied Biosystems, Foster City, CA, USA) as described previously [ 20].
