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Deoxyribonucleic acid was extracted from paraffin-embedded tissue sections, and genotype was determined using the Taqman method.
The current research was undertaken to examine the association between genetic variations in DNA repair and pancreatic cancer risk.We analyzed 9 single nucleotide polymorphisms of 7 DNA repair genes (LIG3, LIG4, OGG1, ATM, POLB, RAD54L, and RECQL) in 734 patients with pancreatic adenocarcinoma and 780 healthy controls using the Taqman method.
Using the TaqMan method and all individuals tested in susceptibility bioassay, the kdr allele frequencies in six Cx.
The same 97 mosquitoes used in the above kdr gene sequencing and AS-PCR method were tested using the Taqman method to determine its sensitivity and specificity.
The kdr allele frequencies were examined in 100 randomly selected adult mosquitoes at generations 6 and 12 of Tangkou population under deltamethrin selection, using the Taqman method.
The six SNPs were genotyped using the Taqman method.
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Therefore, for kdr allele frequency examination described in subsequent sections, we used the TaqMan method.
Genomic DNA was extracted from blood samples of the Bruneck study subjects, and genotypes were determined using the TaqMan genotyping method.
Genotyping was performed in all subjects using the TaqMan probe method.
rs7903146 was genotyped using the TaqMan PCR method (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's instructions.
Genotyping was performed using the TaqMan Probe method with an ABI 7500 Real-time PCR system (Applied Biosystems, Foster City, CA, USA).
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