Sentence examples for using the taqman approach from inspiring English sources

RT-PCR was carried out using the TaqMan approach on a StepOnePlus instrument (Applied Biosystems, USA).

cDNA was subjected to real-time PCR analysis in an ABI PRISM 7700 Sequence Detector (Applied Biosystems Inc., Foster City, CA, USA) using the TaqMan approach.

We therefore examined the sensitivity and accuracy of this approach using the TaqMan real-time PCR data as the "reference" data sets.

Genotypes were determined using the Taqman allelic discrimination assay.

To quantify the miR156 and miR172 expression, PCR was performed using the TaqMan Fast Universal PCR Master Mix (Applied Biosystem).

The amplification efficiencies of both primer sets were assessed using the TaqMan and SYBR Green systems.

Reverse transcription was performed using the TaqMan Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) and a Gene Amp PCR System 9700 (Applied Biosystems) was used to generate cDNA.

We genotyped SNPs using the TaqMan assay.

PCR products were amplified from each cDNA sample using the TaqMan MicroRNA Assay together with the TaqMan® Universal PCR Master Mix.

The HYPEST sample was genotyped using Taqman, individuals from Whitehall II and the ELSA study were also genotyped using the Taqman assay at Geneservice UK.

Quantitative 384 well TaqMan Low Density Array real-time PCR was run on the ABI PRISM 7900 System using the TaqMan Universal PCR Master Mix.