Sentence examples for using the taqman Reverse from inspiring English sources
Single strand cDNA was synthesized by using the 'TaqMan Reverse Transcription Reagents' (Applied Biosystems, Inc., Loughborough, UK) and oligo dT primer according to the manufacturer's instructions.
Reverse transcription was performed using the TaqMan Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) and a Gene Amp PCR System 9700 (Applied Biosystems) was used to generate cDNA.
RNA samples were then reverse transcribed using the TaqMan Reverse Transcription Reagents (Applied Biosystems, Part No. N808-0234), according to the manufacturers instructions.
Reverse transcription was performed for RNA viruses using the TaqMan Reverse Transcription Reagents kit (Roche) and random hexanucleotides as per manufacturer's instructions.
cDNA were generated from 1 µg of total RNA using the Taqman reverse transcription kit (Applied Biosystems) according to manufacturer's instructions in a total volume of 100 µl.
Total RNA (1 µg) was reversed transcribed in 50 µl reactions using the TaqMan Reverse Transcription kit (Applied Biosystems, Foster City, USA), according to the manufacturer's instructions.
Five µl of total RNA was reverse transcribed using the TaqMan miRNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA) and miRNA-specific stem-loop primers (part of TaqMan miRNA assay kit; Applied Biosystems).
For microRNA based RT-PCR assays, 2.5 µL of enriched small RNAs from plasma samples were reverse transcribed using the TaqMan MicroRNA Reverse Transciption Kit (Applied Biosystems, San Diego, CA) according to manufacturer's instructions in a total reaction volume of 7.5 µL.
Briefly, RNA samples were reverse transcribed using the Taqman MicroRNA reverse transcription kit (Applied Biosystems, Applera, Italy) and primers specific for each miRNA (hsa-miR-17-5p, hsa-miR-20a, hsa-miR-9, hsa-miR-9*), according to manufacturer's instructions.
Input RNA was reverse transcribed using the TaqMan miRNA Reverse Transcription kit and miRNA-specific stem-loop primers (Life Technologies, Madrid, Spain).
15 ng of total RNAs were reverse transcribed using the Taqman miRNA reverse transcription kit with Taqman miRNA Multiplex RT assays (human pool) (Applied Biosystems, Foster City, CA, USA).
