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Clones representing each OTU0.97 for each library were analyzed using the tRFLP procedures described above with the threshold set at 100 RFU.
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Additional TRFLP fragment size predictions were generated using the TRFLP analysis program APLAUS + [ 81].
This was achieved using the following procedure.
Prepared using the procedure for making 14x.
France, too, has used the same procedure.
Most national media pollsters use the same procedure.
We thus used the following procedure.
Poly As use the same procedure.
Use the same procedure.
Use the following procedure for defrosting.
use the same procedure every time.
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