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High affinity DNA aptamers against anatoxin-a (ATX), the smallest potent neurotoxin (Mol. Wt, 165.23 Da) were selected and identified in vitro using the systematic evolution of ligands by exponential enrichment (SELEX) approach.
Aptamers have been selected using the systematic evolution of ligands by the exponential enrichment (SELEX) process.
This in vitro selection procedure is usually performed using the Systematic Evolution of Ligands by Exponential enrichment (SELEX) process (11, 12).
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We describe here the use of the systematic evolution of ligands by exponential enrichment (SELEX) technique to develop a sandwich ELISA method based on an MPT64 antibody aptamer for the diagnosis of PTB.
High affinity aptamers for specific target molecules can be isolated from a library of randomized sequences in vitro using the SELEX (systematic evolution of ligands by exponential enrichment) process [21].
Input sequences are screened for consensus binding sequences for the SR proteins SF2/ASF, SC35, SRp40 and SRp55, developed using the SELEX (systematic evolution of ligands by exponential enrichment) procedure [ 11, 21, 22].
We describe here an in vitro technique that directly used live bacterial cells and the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) strategy.
The systematic evolution of ligands by exponential enrichment which uses iterative screening of nucleic acid libraries is a popular experimental technique.
We carried out computational simulations of aptamer-protein interactions by using ZDOCK and ZRANK functions in Discovery Studio 3.5 starting from the available information of aptamers generated through the systematic evolution of ligands by exponential enrichment (SELEX) in the literature.
Long-term measurements show the systematic evolution of the radiation pattern of one of the youngest neutron stars known.
In addition we used the systematic review filter "systematic".
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