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Moreover, additional N-methylation derivatives were also obtained using the synthetic sequence.
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The ability of rhPCR to discriminate single base mismatches was first studied using the synthetic 103mer target sequence described above.
This was compared to an abundance analysis of the metagenomic sequences using the synthetic metagenome as reference set.
The synthetic sequence was developed using two palladium mediated reactions including, at the end of the synthesis, a direct (het)arylannulation, which led to the desired heterocycles.
Integration of the synthetic sequence, mediated by the presence of the bchC and pufQ sequences, was confirmed by colony PCR using the CheckF and CheckR primers detailed above.
GMG was firstly purified by one of the authors in 2000 and using a synthetic sequence the structure of this glucosinolate extracted from M. oleifera seeds was unequivocally ascribed and fully characterized by H and C NMR and mass spectrometry [ 10, 11].
We use the synthetic modeling approach.
Expression of GM-CSF in JS7 in the position of Nef was achieved using a synthetic sequence and standard recombinant DNA technology.
The synthetic sequences are subjectively evaluated using the DSCQS methodology [23], provided by the International Telecommunication Union through the recommendation ITU-R BT.500-11, for a subjective assessment of the quality of videos.
The ARE- luc reporter was constructed using entirely synthetic sequences designed to identify ARE activators operating only through a Nrf2-dependent mechanism.
These form a useful resource for benchmarking, but since a rather small fraction of functional sites are likely to be annotated in this database, the benchmark here uses synthetic sequence that contains embedded REDfly footprints as well as synthetic samples from PWMs, as described in Materials and Methods.
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