Sentence examples for using the supernatant samples from inspiring English sources
Protein levels of the cytokine interleukin (IL -8 were measured usIL -8he supernatant samples.
The genome-wide DNA methylation levels were quantified by analyzing mapped sequencing reads as methylation peaks, using the supernatant of each sample as standard background, and are referred to as total number of peaks (Supplementary Table S4).
Such targets are easily detected by specific antibodies via enzyme-linked immunosorbent assay (ELISA) or dot blot analysis using the supernatant or sediments of the blood sample.
All the supernatant samples were stored at −20°C and were brought to room temperature before use.
The supernatant sample was used to detect alpha-hemolysin, while the cell pellet sample was used to detect SaeP.
We measured the protein abundance of the cytokine IL-8 using the basolateral supernatant from all 12 samples.
The pellet fraction was dissolved in the same homogenization buffer as above without detergent (10× volume of the tissue weight) and then treated like and used according to supernatant samples.
The requirement to deplete tissue culture supernatant derived RNA was investigated using two 100 ml supernatant samples.
Sytox Blue dead cell stain (Molecular Probes, the Netherlands) was used to discriminate between live and dead cells.> The production of pro- and anti-inflammatory cytokines was assessed by enzyme-linked immunosorbent assay (ELISA) in cell-free supernatant samples using the Human Inflammatory 5-Plex Panel (Invitrogen, USA).
Methodological differences may account for our different, yet not contradictory findings: whereas many studies have employed single-cytokine ELISA tests, used plasma or culture supernatant samples, or had cohorts of newly diagnosed disease, we employed a multiplex assay, used serum samples, and had a cohort of chronic disease.
The amount of cAMP and PGE2 in the cell supernatant samples was quantified using an Alpha screen cAMP Assay Kit and PGE2 Assay Kit (PerkinElmer), respectively, according to the manufacturer's instructions.
