Sentence examples similar to using the subsequent methods from inspiring English sources

However, in the case of the analogous solid solutions prepared by us using the mechanical activation method and subsequent isothermal annealing [43], the structural transformation from rhombohedral to cubic symmetry occurred within the region of x = 0.4 0.7.

A subsequent larger RCT using the same methods with the same surfactant preparation and study population failed to show any benefits in oxygenation, mortality, length of ICU stay or duration of mechanical ventilation [ 40].

Synthesis of cDNA and subsequent PCR was performed using the standard methods.

Total RNA was isolated from heads of 10-day oldfemales using the TRIzol method (Invitrogen), and subsequent cDNAand QPCR experiments were performed as described previously [ 23].

Relative expression levels were calculated with subsequent ΔCT values that were assessed using the comparative CT method with subsequent ΔCT values normalized to the housekeeping genes HPRT and GAPDH.

Subsequent volumetric estimates using the Cavalieri method confirmed these findings.

These breakpoints were again used to inform subsequent methods of GC detection.

Subsequent comparisons were performed using the LSD method.

Because the detection of microtubule destabilization by immunofluorescence proved more robust than alterations in YFP fluorescence, all subsequent studies were performed using the former method.

Microdialysate collection vials were changed every 30 min for a period of 5 h and stored at −80°C for subsequent analysis by HPLC using the same method as for brain microdialysates (see below).

This review traces the origins of the mixed block/event-related design from conceptual precursors to a seminal paper and on to subsequent studies using the method.